高表达vimentin的巨噬细胞在肝细胞癌免疫抑制中的关键作用_abio生物试剂品牌网
Vimentin结构. Schematic representation of a vimentin filament from a monomer to its assembly into a mature filament. The vimentin monomer is composed of a central a-helical domAIn flanked by an unstructured head and tail domain. On the central domain, vimentin possesses a rod II domain with lectin-like properties.
重要发现：Vimentin高表达（VIM+）巨噬细胞通过分泌IL-1β激活Treg细胞的免疫抑制功能
复旦大学的王红阳/陈磊教授团队于2024年9月在Nature Cancer （IF：23.5）上发表了题为“Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinoma”的研究性文章。该文绘制了HCC单细胞空间蛋白图谱，解析了HCC肿瘤间空间异质性，建立了预后相关的干细胞癌（HCC）空间分型（Spatial Pattern），并整合多组学和体外实验揭示了Vimentin高表达的巨噬细胞与Treg细胞的空间互作促进HCC的疾病进展。
该项研究通过开展了单细胞测序和空间转录组分析，揭示了VIM+巨噬细胞的基因表达特征，发现了VIM+巨噬细胞和Treg之间基因表达的高度关联性，提示了VIM+巨噬细胞可能作用于Treg，从而增强免疫抑制。配受体分析进一步揭示了VIM+巨噬细胞或许通过白介素-1β（IL-1β）和干扰素-γ（IFN-γ）参与对Treg细胞的调控。随后，作者利用流式细胞仪分离了VIM+和VIM低表达的巨噬细胞，并检测了其中IL-1β的表达水平。结果表明，在不同类型的巨噬细胞中，VIM+的巨噬细胞显著高表达IL-1β，细胞共培养实验发现了VIM+巨噬细胞促进Treg分泌IL-10，而加入IL-1β抗体可显著缓解作用效果。
斯达特S-RMab®
Vimentin Recombinant Rabbit mAb (SDT-029-120) （货号：S0B2254）抗体是您Vimentin 研究中的有力帮手。
数据验证：
WB免疫印记
WB result of Vimentin Rabbit mAb
Primary antibody: Vimentin Rabbit mAb at 1/500 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 57 kDa
IHC 免疫组化
斯达特S-RMab® 系列抗体赋能生命科学和病理研究
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Vimentin antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
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